MS-Fit Search Results


Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-109
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 30000 Da) selects 44484 entries.
pI search (6.00 - 10.00) selects 66491 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 2478 entries.
Pre searches select 2478 entries.

Data Set 1 Results

MS-Fit search selects 67 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/150(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16708 (5)38.05.3-37.145.3 22392/8.4HUMANTransgelin 2 (SM22-alpha homolog)
25275 (3)17.03.3-25.381.7 26541/8.1MOUSERas-related protein Rab-33A (Small GTP-binding protein S10)
35265 (3)17.03.3-25.381.7 26593/8.1HUMANRas-related protein Rab-33A (Small GTP-binding protein S10)
44545 (3)12.03.3-20.536.8 29347/8.7MOUSESynaptonemal complex protein 3 (SCP-3 protein)
53254 (2)13.02.7-33.256.3 23850/6.9HUMANGlycolipid transfer protein (GLTP)
63255 (3)15.03.3-21.257.1 21461/9.4MOUSEDNA BINDING PROTEIN RFX3
72758 (5)17.05.3-22.935.5 26678/6.4MOUSERas-related protein Rab-23 (Rab-15)
82674 (2)12.02.7-43.822.0 22349/8.7HUMANProtein C20orf102
92655 (3)26.03.3-31.622.7 25970/7.7MOUSEGlutathione S-transferase Mu 1 (GST class-mu 1) (Glutathione S-transferase GT8.7) (pmGT10) (GST 1-1)
102565 (3)20.03.3-19.863.7 22471/6.8MOUSENeuronal protein NP25 (Transgelin 3)
54656........................................................xx.......x...............x....x..............x.......x.....x..................................
107479......................x.........x..................................................................................x..x..............x................
74910......................x.........x..................................................................................x..x..............x................
82981................x.....x............................................................................................x..x......x........................
32578....................................x..x.....................................x...............................................................x........
1352...x..........................................................................x.......x..x.x..........................................................
50797.................x.................................x....x.x...........x..x..............................................................x....x........
67485.......................x..............................................................x..x......................x.....................................
26623......................x..................................x......................x.x..............................................x....................
84973.x..........x.............................................x......................x....x...............................................................


Detailed Results

1. 8/150 matches (5%).
Acc. #: Species: HUMAN Name: Transgelin 2 (SM22-alpha homolog)
Index: MW: 22392 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1218.55501218.6265-591Met-ox 1721820(K)NVIGLQMGTNR(G)
1231.60991231.6833-601Met-ox 1281390(R)TLMNLGGLAVAR(D)
1279.58821279.5919-2.91611710(R) (N)
1383.60851383.6149-4.71831960(R) (Q)
1415.53031415.6048-532Met-ox 1831960(R)GASQAGMTGYGMPR(Q)
1594.66191594.7178-351401530(R) (K)
1677.70821677.7906-49pyroGlu 1Met-ox 891020(K)QMEQISQFLQAAER(Y)
1694.75971694.8172-341Met-ox 891020(K)QMEQISQFLQAAER(Y)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 38% (76/199AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/150 matches (3%).
Acc. #: Species: MOUSE Name: Ras-related protein Rab-33A (Small GTP-binding protein S10)
Index: MW: 26541 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1042.51491042.46684656640(R) (T)
1100.44611100.4835-341Met-ox 1011080(K)SMVEHYYR(N)
1694.75971694.8212-361691830(K) (D)
1710.72181710.8161-551Met-ox 1691830(K)FADAHNMLLFETSAK(D)
2131.91192132.0123-4756741(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 17% (42/237AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/150 matches (3%).
Acc. #: Species: HUMAN Name: Ras-related protein Rab-33A (Small GTP-binding protein S10)
Index: MW: 26593 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1042.51491042.46684656640(R) (T)
1100.44611100.4835-341Met-ox 1011080(K)SMVEHYYR(N)
1694.75971694.8212-361691830(K) (D)
1710.72181710.8161-551Met-ox 1691830(K)FADAHNMLLFETSAK(D)
2131.91192132.0123-4756741(R) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (42/237AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/150 matches (3%).
Acc. #: Species: MOUSE Name: Synaptonemal complex protein 3 (SCP-3 protein)
Index: MW: 29347 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1003.47571003.5060-301311380(K) (L)
1042.51491042.5686-511952020(K) (S)
1694.75971694.7664-4.02332460(K) (K)
1710.72181710.7613-231Met-ox 2332460(K)VMMETQQQEMANVR(K)
1838.84331838.8563-7.01Met-ox 2332471(K)VMMETQQQEMANVRK(S)
1838.84331838.8563-7.01Met-ox 2322461(K)KVMMETQQQEMANVR(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (32/254AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/150 matches (2%).
Acc. #: Species: HUMAN Name: Glycolipid transfer protein (GLTP)
Index: MW: 23850 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1110.45611110.4930-3379870(K) (V)
1126.42931126.4879-521Met-ox 79870(K)EMYGAEWPK(V)
1355.74521355.73636.51471580(K) (S)
2367.13702367.2654-541391581(K) (S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 13% (29/209AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 5/150 matches (3%).
Acc. #: Species: MOUSE Name: DNA BINDING PROTEIN RFX3
Index: MW: 21461 Da pI: 9.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
870.4273870.414415pyroGlu 1401460(R)QQPMQQK(Q)
1357.65301357.6653-9.01091191(R) (V)
1415.53031415.6122-581291390(R) (Q)
1431.54781431.6071-411Met-ox 1291390(R)LQEDMQYMAMR(Q)
1447.58351447.6020-132Met-ox 1291390(R)LQEDMQYMAMR(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (29/189AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 8/150 matches (5%).
Acc. #: Species: MOUSE Name: Ras-related protein Rab-23 (Rab-15)
Index: MW: 26678 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1008.43261008.4461-132292370(R) (-)
1179.53911179.5897-431541630(K) (Y)
1218.55501218.5928-31AcetN 1110(-)LEEDMEVAIK(M)
1234.59181234.58773.3AcetN 1Met-ox 1110(-)LEEDMEVAIK(M)
1307.61091307.6227-9.01110(-) (M)
1323.56681323.6176-381Met-ox 1110(-)MLEEDMEVAIK(M)
2230.05292230.1463-42AcetN 1221(-)LEEDMEVAIKMVVVGNGAVGK(S)
2367.13702367.1610-103Met-ox 1221(-)MLEEDMEVAIKMVVVGNGAVGK(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (41/237AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 4/150 matches (2%).
Acc. #: Species: HUMAN Name: Protein C20orf102
Index: MW: 22349 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.48661045.5431-5491990(K) (A)
1415.53031415.5935-4554650(R) (G)
1431.54781431.5884-281Met-ox 54650(R)TGEDVEMACSFR(G)
1690.73781690.8189-4886991(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (26/204AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 5/150 matches (3%).
Acc. #: Species: MOUSE Name: Glutathione S-transferase Mu 1 (GST class-mu 1) (Glutathione S-transferase GT8.7) (pmGT10) (GST 1-1)
Index: MW: 25970 Da pI: 7.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1042.51491042.5355-201942021(K) (Y)
1231.60991231.6397-241Met-ox 1351441(K)MKLYSEFLGK(R)
1379.65951379.6968-271110(-) (G)
1390.60741390.6637-40971080(R) (M)
1993.89841993.9911-461531680(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (58/218AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 5/150 matches (3%).
Acc. #: Species: MOUSE Name: Neuronal protein NP25 (Transgelin 3)
Index: MW: 22471 Da pI: 6.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4164864.4579-48991060(K) (T)
967.4609967.43732422290(K) (L)
1234.59181234.6142-18pyroGlu 89980(K)QMEQISQFLK(A)
1383.60851383.6149-4.71831960(K) (Q)
1415.53031415.6048-532Met-ox 1831960(K)GASQAGMTGYGMPR(Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (40/199AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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